| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
19/09/2018 |
|
Data da última atualização: |
28/09/2018 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
SILVA, L. L.; PESTANA, K. N.; FERREIRA, C. F.; OLIVEIRA, S. A. S. de. |
|
Afiliação: |
LEANDRO L. SILVA, UFRB; KÁTIA N. PESTANA; CLAUDIA FORTES FERREIRA, CNPMF; SAULO ALVES SANTOS DE OLIVEIRA, CNPMF. |
|
Título: |
Differentiation of phylogenetic lineages within the 'Colletotrichum gloeosporioides species complex' associated with cassava anthracnose disease by PCR-RFLP. |
|
Ano de publicação: |
2018 |
|
Fonte/Imprenta: |
Tropical Plant Pathology, v.43, p.194-20, 2018. |
|
ISSN: |
1983-2052 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Different species were found associated with cassava anthracnose, considered a major disease of this crop. The correct identification of the causal agent is a first step for defining appropriate control strategies, such as resistant varieties. In silico analyses used sequences of six genomic regions of ex-type specimens from the ?Colletotrichum gloeosporioides species complex? (C.g.SC) and 21 restriction enzymes to identify phylogenetic lineages. The three best combinations of region/enzymes were validated in 18 Colletotrichum spp. isolates from cassava. Dendrograms for in silico PCR-RFLP for CAL, ITS and TUB2 showed considerable agreement with the phylogenetic analysis of each genomic region; however, the CAL gene presented greater discriminatory power. Since the band patterns from in gel analysis were almost the same as expected for the in silico analysis for the CAL region, a new approach was proposed based on the combined data from these two methodologies, allowing the differentiation of five phylogenetic lineages within the C.g.SC (C. tropicale; C. fructicola; C. siamense; C. gloeosporioides sensu stricto and C. theobromicola), and one outside of this complex (C. cliviae). This evaluation showed to be a reliable technique for preliminary identification of species prior to sequencing. |
|
Thesagro: |
Mandioca. |
|
Thesaurus Nal: |
Cassava. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 01935naa a2200193 a 4500
001 2096059
005 2018-09-28
008 2018 bl uuuu u00u1 u #d
022 $a1983-2052
100 1 $aSILVA, L. L.
245 $aDifferentiation of phylogenetic lineages within the 'Colletotrichum gloeosporioides species complex' associated with cassava anthracnose disease by PCR-RFLP.$h[electronic resource]
260 $c2018
520 $aDifferent species were found associated with cassava anthracnose, considered a major disease of this crop. The correct identification of the causal agent is a first step for defining appropriate control strategies, such as resistant varieties. In silico analyses used sequences of six genomic regions of ex-type specimens from the ?Colletotrichum gloeosporioides species complex? (C.g.SC) and 21 restriction enzymes to identify phylogenetic lineages. The three best combinations of region/enzymes were validated in 18 Colletotrichum spp. isolates from cassava. Dendrograms for in silico PCR-RFLP for CAL, ITS and TUB2 showed considerable agreement with the phylogenetic analysis of each genomic region; however, the CAL gene presented greater discriminatory power. Since the band patterns from in gel analysis were almost the same as expected for the in silico analysis for the CAL region, a new approach was proposed based on the combined data from these two methodologies, allowing the differentiation of five phylogenetic lineages within the C.g.SC (C. tropicale; C. fructicola; C. siamense; C. gloeosporioides sensu stricto and C. theobromicola), and one outside of this complex (C. cliviae). This evaluation showed to be a reliable technique for preliminary identification of species prior to sequencing.
650 $aCassava
650 $aMandioca
700 1 $aPESTANA, K. N.
700 1 $aFERREIRA, C. F.
700 1 $aOLIVEIRA, S. A. S. de
773 $tTropical Plant Pathology$gv.43, p.194-20, 2018.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 2 | |
| 1. |  | POLIDORO, J. C.; MENDONÇA-SANTOS, M. de L.; LUMBRERAS, J. F.; COELHO, M. R.; CARVALHO FILHO, A. de; MOTTA, P. E. F. da; CARVALHO JUNIOR, W. de; ARAUJO FILHO, J. C. de; CURCIO, G. R.; CORREIA, J. R.; MARTINS, E. de S.; SPERA, S. T.; OLIVEIRA, S. R. de M.; BOLFE, E. L.; MANZATTO, C. V.; TOSTO, S. G.; VENTURIERI, A.; SA, I. B.; OLIVEIRA, V. A. de; SHINZATO, E.; ANJOS, L. H. C. dos; VALLADARES, G. S.; RIBEIRO, J. L.; MEDEIROS, P. S. C. de; MOREIRA, F. M. de S.; SILVA, L. S. L.; SEQUINATTO, L.; AGLIO, M. L. D.; DART, R. de O. Programa Nacional de Solos do Brasil (PronaSolos). Rio de Janeiro: Embrapa Solos, 2016. 53 p. (Embrapa Solos. Documentos, 183).| Biblioteca(s): Embrapa Agrossilvipastoril; Embrapa Amazônia Oriental; Embrapa Cocais; Embrapa Florestas; Embrapa Meio Ambiente; Embrapa Semiárido; Embrapa Solos. |
|    |
| 2. | | MORALES, M. M.; SARTORI, W. W.; SILVA, B. R.; SPERA, S. T.; MENDES, A. B. D.; AMBROSIO-ALBUQUERQUE, E. P. Wood vinegar: chemical characteristics, phytotoxic effects, and impacts on greenhouse gas emissions. Nativa, v. 10, n. 3, p. 400-409, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Agrossilvipastoril; Embrapa Florestas. |
| |
| Registros recuperados : 2 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
